Getting Started¶
Installation¶
pip install git+https://github.com/scmgl-kaist/sceleto.git
With optional dependencies:
pip install "sceleto[all] @ git+https://github.com/scmgl-kaist/sceleto.git"
Marker Discovery¶
import scanpy as sc
import sceleto as scl
adata = sc.read_h5ad("your_data.h5ad")
# Graph-based marker detection
result = scl.markers.marker(adata, groupby="cell_type")
# Per-group marker genes
result.markers # {'T_cell': ['CD3D', 'CD3E', ...], ...}
# Dotplot
scl.dotplot(adata, result.markers, "cell_type")
Cross-resolution Hierarchy¶
m1 = scl.markers.marker(adata, groupby="leiden_0.1")
m2 = scl.markers.marker(adata, groupby="leiden_0.5")
m3 = scl.markers.marker(adata, groupby="leiden_1.0")
res = scl.markers.hierarchy(adata, [m1, m2, m3])
res.compare_markers("0") # static heatmap
res.interactive_viewer(adata, save="viewer.html") # interactive HTML
Gene Network¶
import sceleto as scl
# Correlation-based gene network for a gene of interest
corr_df = scl.network.build_corr_matrix(
{"CD4": adata_cd4, "CD8": adata_cd8},
gene="CD3D",
)
top_genes = scl.network.select_top_genes(corr_df, top_n=10, exclude_gene="CD3D")
feat = scl.network.build_feature_matrix(top_genes, corr_df)
G = scl.network.build_gene_network(feat, k=5)
scl.network.plot_network(G, corr_df)
# One-shot from PANGEA pre-computed correlation DB
corr_df, feat, G = scl.network.corr_pangea("CD3D", data_dir="path/to/pangea_corr/")
Cell Type Annotation¶
import sceleto as scl
# PANGEA-based cell type annotation
pred = scl.annotation.cellannotator(adata)
meta = scl.annotation.metaannotator(pred)